Efficient Transfection of Chicken Blastoderms in vivo by Lipofection and Electroporation Using Green Fluorescent Protein Gene as a Marker
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چکیده
منابع مشابه
Transient transfection of Cryptosporidium parvum using green fluorescent protein (GFP) as a marker.
Cryptosporidium parvum is a protozoan parasite that infects a variety of mammals. The parasite has been shown to harbor a dsRNA virus (CPV) and in the present study, we have developed a CPV transient transfection system for this parasite by using green fluorescent protein (GFP) to replace the partial gene encoding region of the larger dsRNA (CPV-L) and the smaller dsRNA (CPV-S) virus. Two viral...
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Transformation of rickettsiae is a recent accomplishment, but utility of this technique is limited due to the paucity of selectable markers suitable for use in this intracellular pathogen. We chose a green fluorescent protein variant optimized for fluorescence under UV lights (GFPUV) as a fluorometric marker and transformed Rickettsia typhi with an rpoB-GFPUV fusion construct. The rickettsiae w...
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A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 100% entrapment of the DNA, DOTMA facilitates fusion of the complex with the plasma membrane of tissue culture cells, resul...
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ژورنال
عنوان ژورنال: Nihon Chikusan Gakkaiho
سال: 2000
ISSN: 1346-907X,1880-8255
DOI: 10.2508/chikusan.71.377